88 research outputs found
Physiological Characterization of Stolon Regression in a Colonial Hydroid
As with many colonial animals, hydractiniid hydroids display a range of morphological variation. Sheet-like forms exhibit feeding
polyps close together with short connecting stolons, whereas runner-like forms have more distant polyps and longer connecting
stolons. These morphological patterns are thought to derive from rates of stolon growth and polyp formation. Here, stolon
regression is identified and characterized as a potential process underlying this variation. Typically, regression can be observed
in a few stolons of a normally growing colony. For detailed studies, many stolons of a colony can be induced to regress by
pharmacological manipulations of reactive oxygen species (e.g. hydrogen peroxide) or reactive nitrogen species (e.g. nitric
oxide). The regression process begins with a cessation of gastrovascular flow to the distal part of the stolon. High levels of
endogenous H2O2 and NO then accumulate in the regressing stolon. Remarkably, exogenous treatments with either H2O2 or an NO
donor equivalently trigger endogenous formation of both H2O2 and NO. Cell death during regression is suggested by both
morphological features, detected by transmission electron microscopy, and DNA fragmentation, detected by TUNEL. Stolon
regression may occur when colonies detect environmental signals that favor continued growth in the same location rather than
outward growth
Developing High Performance Computing Resources for Teaching Cluster and Grid Computing courses
High-Performance Computing (HPC) and the ability to process large amounts of data are of
paramount importance for UK business and economy as outlined by Rt Hon David Willetts
MP at the HPC and Big Data conference in February 2014. However there is a shortage of
skills and available training in HPC to prepare and expand the workforce for the HPC and
Big Data research and development. Currently, HPC skills are acquired mainly by students
and staff taking part in HPC-related research projects, MSc courses, and at the dedicated
training centres such as Edinburgh Universityâs EPCC. There are few UK universities teaching
the HPC, Clusters and Grid Computing courses at the undergraduate level. To address the
issue of skills shortages in the HPC it is essential to provide teaching and training as part of
both postgraduate and undergraduate courses. The design and development of such courses is
challenging since the technologies and software in the fields of large scale distributed systems
such as Cluster, Cloud and Grid computing are undergoing continuous change. The students
completing the HPC courses should be proficient in these evolving technologies and equipped
with practical and theoretical skills for future jobs in this fast developing area.
In this paper we present our experience in developing the HPC, Cluster and Grid modules
including a review of existing HPC courses offered at the UK universities. The topics covered in
the modules are described, as well as the coursework projects based on practical laboratory work.
We conclude with an evaluation based on our experience over the last ten years in developing
and delivering the HPC modules on the undergraduate courses, with suggestions for future work
Redox Signaling in Colonial Hydroids: Many Pathways for Peroxide
Studies of mitochondrial redox signaling predict that
the colonial hydroids Eirene viridula and Podocoryna
carnea should respond to manipulations of reactive oxygen
species (ROS). Both species encrust surfaces with feeding
polyps connected by networks of stolons; P. carnea is more
âsheet-likeâ with closely spaced polyps and short stolons,
while E. viridula is more ârunner-likeâ with widely spaced
polyps and long stolons. Treatment with the chemical antioxidant
vitamin C diminishes ROS in mitochondrion-rich
epitheliomuscular cells (EMCs) and produces phenotypic
effects (sheet-like growth) similar to uncouplers of
oxidative phosphorylation. In peripheral stolon tips,
treatment with vitamin C triggers a dramatic increase of
ROS that is followed by tissue death and stolon regression.
The enzymatic anti-oxidant catalase is probably not taken
up by the colony but, rather, converts hydrogen peroxide
in the medium to water and oxygen. Exogenous catalase
does not affect ROS in mitochondrion-rich EMCs, but
does increase the amounts of ROS emitted from
peripheral stolons, resulting in rapid, runner-like growth.
Treatment with exogenous hydrogen peroxide increases
ROS levels in stolon tips and results in somewhat faster
colony growth. Finally, untreated colonies of E. viridula
exhibit higher levels of ROS in stolon tips than untreated
colonies of P. carnea. ROS may participate in a number of
putative signaling pathways: (1) high levels of ROS may
trigger cell and tissue death in peripheral stolon tips; (2)
more moderate levels of ROS in stolon tips may trigger
outward growth, inhibit branching and, possibly, mediate
the redox signaling of mitochondrion-rich EMCs; and (3)
ROS may have an extra-colony function, perhaps in
suppressing the growth of bacteria
Investigating the perceived versus actual gastrointestinal nematode challenge on extensive sheep farms
Extensive farming systems form an integral part of sheep production systems across Europe. However, with innate production handicaps, declining sheep numbers and narrow economic margins, production is becoming increasingly challenging threatening the future sustainability of the industry. Gastrointestinal nematodes (GINs) are a significant cause of production losses to the global sheep industry, with well-established resistance to the major anthelmintic groups. Traditionally, extensive farming systems are not thought to have a significant parasite challenge compared with intensive farms, but there is a need to identify the scale and importance of GINs on extensive farms to inform the need for sustainable control strategies. In this study, a questionnaire of extensive farmers (n=34) was conducted and parasitological data were collected from nine study farms to investigate the perceived versus actual GIN and anthelmintic resistance challenge faced by extensive farms. The results showed a production-limiting challenge on most farms, with a higher GIN challenge observed on improved pastures. Furthermore, over half of the extensive farmers perceived anthelmintic resistance to be a greater problem for intensive farmers, with only 20% of respondents reporting known anthelmintic resistance. However, all study farms had evidence of resistance to at least one group of anthelmintics. Consequently, this study has demonstrated that despite the traditional perception of parasitism on extensive farms, there is a need to increasingly consider its impact and take a proactive approach to sustainable control, with solutions tailored to their unique management
Focus on environmental risks and migration: causes and consequences
Environmental change poses risks to societies, including disrupting social and economic systems such as migration. At the same time, migration is an effective adaptation to environmental and other risks. We review novel science on interactions between migration, environmental risks and climate change. We highlight emergent findings, including how dominant flows of rural to urban migration mean that populations are exposed to new risks within destination areas and the requirement for urban sustainability. We highlight the issue of lack of mobility as a major issue limiting the effectiveness of migration as an adaptation strategy and leading to potentially trapped populations. The paper presents scenarios of future migration that show both displacement and trapped populations over the incoming decades. Papers in the special issue bring new insights from demography, human geography, political science and environmental science to this emerging field
Acute blood biomarker profiles predict cognitive deficits 6 and 12 months after COVID-19 hospitalization
Post-COVID cognitive deficits, including âbrain fogâ, are clinically complex, with both objective and subjective components. They are common and debilitating, and can affect the ability to work, yet their biological underpinnings remain unknown. In this prospective cohort study of 1,837 adults hospitalized with COVID-19, we identified two distinct biomarker profiles measured during the acute admission, which predict cognitive outcomes 6 and 12 months after COVID-19. A first profile links elevated fibrinogen relative to C-reactive protein with both objective and subjective cognitive deficits. A second profile links elevated D-dimer relative to C-reactive protein with subjective cognitive deficits and occupational impact. This second profile was mediated by fatigue and shortness of breath. Neither profile was significantly mediated by depression or anxiety. Results were robust across secondary analyses. They were replicated, and their specificity to COVID-19 tested, in a large-scale electronic health records dataset. These findings provide insights into the heterogeneous biology of post-COVID cognitive deficits
The Vicious Circle of Post-Soviet Neopatrimonialism in Russia
Published online: 10 Aug 2015, journal issue (vol.32, N5) appeared in 2016Since the collapse of Communism, Russia and some other post-Soviet states have attempted to pursue socio-economic reforms while relying upon the political institutions of neopatrimonialism. This politico-economic order was established to serve the interests of ruling groups and establish the major features of states, political regimes, and market economies. It provided numerous negative incentives for governing the economy and the state due to the unconstrained rent seeking behavior of major actors. Policy reform programs discovered these institutions to be incompatible with the priorities of modernization, and efforts to resolve these contradictions through a number of partial and compromise solutions often worsened the situation vis-Ă -vis preservation of the status quo. The ruling groups lack incentives for institutional changes, which could undermine their political and economic dominance, and are caught in a vicious circle: reforms often result in minor returns or cause unintended and undesired consequences. What are the possible domestic and international incentives to reject the political institutions of neopatrimonialism in post-Soviet states and replace them with inclusive economic and political ones?Peer reviewe
Nanoscale control of Ag nanostructures for plasmonic fluorescence enhancement of near-infrared dyes
Potential utilization of proteins for early detection and diagnosis of various diseases has drawn considerable interest in the development of protein-based detection techniques. Metal induced fluorescence enhancement offers the possibility of increasing the sensitivity of protein detection in clinical applications. We report the use of tunable plasmonic silver nanostructures for the fluorescence enhancement of a near-infrared (NIR) dye (Alexa Fluor 790). Extensive fluorescence enhancement of âŒ2 orders of magnitude is obtained by the nanoscale control of the Ag nanostructure dimensions and interparticle distance. These Ag nanostructures also enhanced fluorescence from a dye with very high quantum yield (7.8 fold for Alexa Fluor 488, quantum efficiency (Qy) = 0.92). A combination of greatly enhanced excitation and an increased radiative decay rate, leading to an associated enhancement of the quantum efficiency leads to the large enhancement. These results show the potential of Ag nanostructures as metal induced fluorescence enhancement (MIFE) substrates for dyes in the NIR âbiological windowâ as well as the visible region. Ag nanostructured arrays fabricated by colloidal lithography thus show great potential for NIR dye-based biosensing applications
Recommended from our members
Risk Factors for Severe Outcomes in Patients With Systemic Vasculitis and COVIDâ19: A Binational, RegistryâBased Cohort Study
Funder: Vifor Pharma; Id: http://dx.doi.org/10.13039/501100006484Objective: COVIDâ19 is a novel infectious disease with a broad spectrum of clinical severity. Patients with systemic vasculitis have an increased risk of serious infections and may be at risk of severe outcomes following COVIDâ19. We undertook this study to establish the risk factors for severe COVIDâ19 outcomes in these patients, including the impact of immunosuppressive therapies. Methods: A multicenter cohort was developed through the participation of centers affiliated with national UK and Ireland vasculitis registries. Clinical characteristics and outcomes are described. Logistic regression was used to evaluate associations between potential risk factors and a severe COVIDâ19 outcome, defined as a requirement for advanced oxygen therapy, a requirement for invasive ventilation, or death. Results: The cohort included 65 patients with systemic vasculitis who developed COVIDâ19 (median age 70 years, 49% women), of whom 25 patients (38%) experienced a severe outcome. Most patients (55 of 65 [85%]) had antineutrophil cytoplasmic antibodyâassociated vasculitis (AAV). Almost all patients required hospitalization (59 of 65 [91%]), 7 patients (11%) were admitted to intensive care, and 18 patients (28%) died. Background glucocorticoid therapy was associated with severe outcomes (adjusted odds ratio [OR] 3.7 [95% confidence interval 1.1â14.9]; P = 0.047), as was comorbid respiratory disease (adjusted OR 7.5 [95% confidence interval 1.9â38.2]; P = 0.006). Vasculitis disease activity and nonglucocorticoid immunosuppressive therapy were not associated with severe outcomes. Conclusion: In patients with systemic vasculitis, glucocorticoid use at presentation and comorbid respiratory disease were associated with severe outcomes in COVIDâ19. These data can inform clinical decisionâmaking relating to the risk of severe COVIDâ19 in this vulnerable patient group
- âŠ